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B. anthracis strains
|
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34 F2
|
Wild-type Sterne; pXO1+, pXO2−
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Laboratory stock
|
|
BCD
|
34 F2 ΔcodY::aphA3, KmR
|
This study
|
|
E. coli strains
|
|
DH5α
|
supE44 Δlac U169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1
|
New England Biolabs
|
|
ER2925
|
ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2
|
New England Biolabs
|
|
Plasmids
|
|
pKS1
|
An integration vector designed for efficient gene inactivation in B. anthracis; KmR, EmR
|
[56]
|
|
pBCD22
|
pKS1 derivative harboring 500 bp fragment upstream and 500 bp fragment downstream of codY flanking aph3A; KmR, EmR
|
This study
|
