Bacterial strains | Descriptiona | Source or reference |
---|---|---|
B. anthracis strains | ||
34 F2 | Wild-type Sterne; pXO1+, pXO2− | Laboratory stock |
BCD | 34 F2 ΔcodY::aphA3, KmR | This study |
E. coli strains | ||
DH5α | supE44 Δlac U169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | New England Biolabs |
ER2925 | ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 | New England Biolabs |
Plasmids | ||
pKS1 | An integration vector designed for efficient gene inactivation in B. anthracis; KmR, EmR | [56] |
pBCD22 | pKS1 derivative harboring 500Â bp fragment upstream and 500Â bp fragment downstream of codY flanking aph3A; KmR, EmR | This study |