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Table 3 Strains and plasmids used in this study

From: Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions

Bacterial strains Descriptiona Source or reference
B. anthracis strains
34 F2 Wild-type Sterne; pXO1+, pXO2 Laboratory stock
BCD 34 F2 ΔcodY::aphA3, KmR This study
E. coli strains
DH5α supE44 Δlac U169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 New England Biolabs
ER2925 ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 New England Biolabs
Plasmids
pKS1 An integration vector designed for efficient gene inactivation in B. anthracis; KmR, EmR [56]
pBCD22 pKS1 derivative harboring 500 bp fragment upstream and 500 bp fragment downstream of codY flanking aph3A; KmR, EmR This study
  1. aKmR, kanamycin resistant; EmR, erythromycin resistant