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Table 1 Bacterial strains and plasmids used in this study

From: Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens

Strain or Plasmid Relevant genotype or characteristics Reference or source
Strains
 P4 Agrobacterium tumefaciens. Isolated from tobacco rhizosphere (La Côte Saint André, France) Mondy et al., 2013
 P4cinI A. tumefaciens; Gmr a, pAtcinI::Gm This study
 P4Rif A. tumefaciens; Rifr Laboratory collection
 P4RifcinI A. tumefaciens; Rifr, Gmr, pAtcinI::Gm This study
 P4RifNC A. tumefaciens; Rifr, Gmr, pAt::Gm This study
 P4RifRIX A. tumefaciens; Rifr, Gmr, pAtΔcinRcinIcinX::Gm This study
 C58.00 A. tumefaciens; Rifr, Smr, cured of the At and Ti plasmids Laboratory collection
 NT1(pZLR4) A. tumefaciens b Gmr, NT1 derived from strain C58, carrying plasmid ZLR4; OC8-HSL biosensor Cha et al., 1998 ; Pipper et al., 1993
 DH5α Escherichia coli; F, Φ 980dlacZ : M15, recA1, endA1, gyrA96, thi-1, hsdR17(rK–, mK+), supE44,relA1, deoR:(lacZYA–argF)U169 Hanahan, 1983
Plasmids
 pGEM®-T Easy Ampr, high copy number Promega Crop., Madison, WI
  1. aabbreviations are as follows: Rifr, Smr, Gmr, Ampr, resistant to rifampicin, streptomycin, gentamicin, and ampicillin, respectively
  2. bThis strain is also a derivative of strain C58