Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens

Strain or Plasmid

Relevant genotype or characteristics

Reference or source

Strains

 P4

Agrobacterium tumefaciens. Isolated from tobacco rhizosphere (La Côte Saint André, France)

Mondy et al., 2013

 P4cinI

A. tumefaciens; Gmr a, pAtcinI::Gm

This study

 P4Rif

A. tumefaciens; Rifr

Laboratory collection

 P4RifcinI

A. tumefaciens; Rifr, Gmr, pAtcinI::Gm

This study

 P4RifNC

A. tumefaciens; Rifr, Gmr, pAt::Gm

This study

 P4RifRIX

A. tumefaciens; Rifr, Gmr, pAtΔcinRcinIcinX::Gm

This study

 C58.00

A. tumefaciens; Rifr, Smr, cured of the At and Ti plasmids

Laboratory collection

 NT1(pZLR4)

A. tumefaciens b Gmr, NT1 derived from strain C58, carrying plasmid ZLR4; OC8-HSL biosensor

Cha et al., 1998 ; Pipper et al., 1993

 DH5α

Escherichia coli; F, Φ 980dlacZ : M15, recA1, endA1, gyrA96, thi-1, hsdR17(rK–, mK+), supE44,relA1, deoR:(lacZYA–argF)U169

Hanahan, 1983

Plasmids

 pGEM®-T Easy

Ampr, high copy number

Promega Crop., Madison, WI

  1. aabbreviations are as follows: Rifr, Smr, Gmr, Ampr, resistant to rifampicin, streptomycin, gentamicin, and ampicillin, respectively
  2. bThis strain is also a derivative of strain C58