Fig. 4

Read depth and percentage of H allele reads in CH5 mutants. a Read depth and percentage of H allele reads per marker across the CH5 genetic map. Markers are ordered according to their position in the linkage group. Linkage groups are shown in numerical order, which was assigned according to linkage group size and may not reflect relative position in the genome. Identified regions showing chromosome duplication (Dupl.) or loss of heterozygosity (LOH) are marked by black arrows. Identified regions in MS45 are as follows: Dupl. LG10–64 NsiI markers located between 0 and 10.93 cM; LOH LG19–80 NsiI markers located between 0 and 32.14 cM. Identified regions in MS51 are as follows: Dupl. LG2–155 NsiI markers located between 190.10 and 308.74 cM; Dupl. LG5–261 NsiI markers located between 0 and 164.69 cM; LOH LG5–235 NsiI markers located between 164.69 and 373.16 cM; Dupl. LG14–179 NsiI markers located between 126.41 and 229.81 cM; LOH LG27–40 NsiI markers located between 0 and 15.99 cM. b and (c) Mean read depth and mean percentage of H allele reads per marker in identified regions compared to the rest of the genome. Significant differences between identified regions and the rest of the genome for a given individual are marked with an asterisk (two-tailed T-test; p < 0.001). Error bars represent ± one standard error