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Table 1 Summary of transcriptome sequence datasets of the BPH reproductive tract

From: Seminal fluid protein genes of the brown planthopper, Nilaparvata lugens

Group

Number of unigenes

Total unigene

37443

MRT expression

33798

MAG expression

19772

Male specific expressed

523

Obtained peptide database

17902

  1. The longest assembled transcripts of each gene were taken as unigenes. Only unigenes with RPKM value larger than 0.3 were counted as expressed. Owing to the accomplishment of the transcriptomic sequencing of the differences between the N. lugens development and sex genes in our previous study [11], we are able to use the predicted SFP genes as reference sequences to map the transcriptomic datasets and to analyze the expression sex-specific genes. The coding sequence (CDS) of each unigene was analyzed using blastx and estscan (3.03). The generated peptide database was used in proteome query