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Fig. 4 | BMC Genomics

Fig. 4

From: Global profiling of alternative RNA splicing events provides insights into molecular differences between various types of hepatocellular carcinoma

Fig. 4

Validation of ASEs dysregulated in HCC. a Overview of the two isoforms encoded by OSBPL6, VPS13A and ZNF692 genes. Exons are depicted in red and the intervening introns are shown as thin black lines (not to scale). The primers used to detect the isoforms by RT-PCR assays are shown in gray and the sizes of the expected amplicons are also indicated. b cDNA obtained from tissues were analyzed by PCR using specific primers to detect both isoforms of the transcripts encoded by the OSBPL6, VPS13A and ZNF692 genes. Capillary electrophoregrams of the PCR reactions are shown. The positions and the amplitude of the detected amplicons are highlighted by red boxes. The positions of the internal markers are also indicated. The Delta PSI values for both the RNA-Seq and experimental validation assays are shown on the right

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