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Fig. 1 | BMC Genomics

Fig. 1

From: Transcriptome sequencing of Mycosphaerella fijiensis during association with Musa acuminata reveals candidate pathogenicity genes

Fig. 1

Validation of RNA-Seq results using RT-qPCR assays. The cytochrome P450 and oligopeptide transporter were normalized against ubiquitin-conjugating enzyme E2 6, and the proteinase and ferric-chelate reductase were normalized against transcription initiation factor TFIID subunit 10. Fold change was calculated using the 2-ΔΔC T method, relative to the Flask #3 sample. Green bars represent expression in infected leaf tissue; blue bars represent expression in mycelial culture. Error bars represent standard error from mean of technical replicates for each sample. Results confirmed the RNA-Seq data that showed higher expression of the cytochrome P450, proteinase, and oligopeptide transporter in infected leaf tissue, whereas the ferric-chelate reductase had lower expression

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