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Fig. 2 | BMC Genomics

Fig. 2

From: Small RNA and degradome profiling reveals miRNA regulation in the seed germination of ancient eudicot Nelumbo nucifera

Fig. 2

Potential novel miRNAs identified in this study. a The secondary structure of novel_ miR_36 precursor was predicted by MFOLD. Mature miRNA is highlighted in red. b Stem-loop RT-PCR analysis of the identified novel miRNAs. Six novel miRNAs (novel_miR_2, novel_miR_13, novel_miR_29, novel_miR_32, novel_miR_36, novel_miR_66) were confirmed via stem-loop RT-PCR. The sizes of the obtained PCR products were approximately 60 bp. Marker indicates a 50 bp DNA Ladder Marker

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