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Fig. 5 | BMC Genomics

Fig. 5

From: Biochemical and cellular characterization of transcription factors binding to the hyperconserved core promoter-associated M4 motif

Fig. 5

The M4 motif is a bipartite composite element, consisting of two independent binding site modules for THAP11 and Ikaros. a DNA ligands used in affinity chromatography: mono-M4 wild-type (WT), mono-M4 mutant (MT), Mod-M4 (the very flanking nucleotides of the M4 motif are modified), F-MT-M4 (the core” RRACTAYR of M4 motif was modified) and R-MT-M4 (the “downstream core” TCCCRRNR of M4 motif were modified). b Immunblot analysis of DNA affinity chromatography experiments using five DNA ligands: Input (Molt4 nuclear extract) and eluates from three columns were resolved by SDS-PAGE in parallel and subjected to Western blotting. Specific antibodies detected the retention of suspected M4 binding factors

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BMC Genomics

ISSN: 1471-2164

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