Fig. 7

Chromatin Immunoprecipitation (ChIP) analysis of NFKB1 (NF-kappa B p50), THAP11 and HCF-1 interaction with M4 genes in Ikaros knockdown cells. Formaldehyde crosslinked chromatin of Molt4 or Molt4-IK-KD cells was sheared by ultrasonication into an average length of 300 to 1000 bps and ultimately subjected to immuno-precipitation assays using a anti-THAP11 antibody, b anti-NF-kappa B-p50 (NFKB1) antibody, c anti HCF-1, or preimmune IgG sera (control). The TF-associated chromatin were analyzed via quantitative real time PCR (qRT-PCR) using primer sets flanking the promoters (M4 motif), and gene body control regions (Gene body) of M4-bearing Skp2, CDC25A, and G9a genes; d the known NFKB1 (NF-kappa-B p50) target genes Vcam-1, HLA-B were also included as control. Enrichment of TF-bound DNA amount were normalized to corresponding amount in input. ChIP results (average of three technical replicates) for NFKB1/NF-kappa B p50 (c), THAP11 (a), and HCF-1 (b) were shown