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Fig. 4 | BMC Genomics

Fig. 4

From: A missense mutation in solute carrier family 12, member 1 (SLC12A1) causes hydrallantois in Japanese Black cattle

Fig. 4

Localization of SLC12A1 protein in fetal kidney of Japanese Black cattle. a, b, c Localization of SLC12A1 in the kidney of fetus of 46-cm crown-rump length (CRL), approximately 160 days of gestation. Sections were immunostained with anti-SLC12A1 antibody (a1, a2, b, c; magenta) and anti-Tamm-Horsfall antibody (a1, a3; green), and counterstained with DAPI (a1, b, c). Images a and b show the renal cortex and medulla, respectively. a1 Distal convoluted tubules are indicated by arrows. b Thick limbs of the loop of Henle are indicated by arrows. Image c shows higher magnification of a distal convoluted tubule (SLC12A1; magenta). Basal membrane side is indicated by dotted-line circle. Scale bars: a, b = 50 μm; c = 10 μm

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