Fig. 6

Identification of a founder individual for hydrallantois in Japanese Black cattle. gDNA was extracted from semen (germ-line cells) from sire_X, the father, maternal grandsire, maternal great-grandsire, and from endometrial cells (somatic cells) derived from the uterus of the mother of sire_X. The gDNA was genotyped using GGP Bovine 26 K BeadChip array and g.62382825G > A using PCR. a, b Non-risk-haplotypes/non-risk-allele carriers, and risk-haplotype/risk-allele carriers are represented with blue-filled symbols and magenta-filled symbols, respectively. Individuals for which we could not determine the haplotype or the allele by genotyping are denoted by empty symbols (ND). c Calibration curve of g.62382825G > A. d Sequence electropherograms G/A allele molar ratio in gDNA from semen (germ-line cells, d2) and gDNA from dermal fibroblast (somatic cells, d3) from sire_X was calculated with the ratio of the peak height of G:A alleles in gDNA of the calibration sample (d1)