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Fig. 2 | BMC Genomics

Fig. 2

From: Transcriptomic analysis reveals sex-specific differences in the expression of Dcl1 and Fis1 genes in the radio-adaptive response of thymocytes to TRP53-mediated apoptosis

Fig. 2

Dissimilar adaptive response of thymocyte apoptosis induced by priming low dose X-ray irradiation in C57BL/6 male and female mice. a Representative results of the Western blotting assay for the expression of caspase-3. HIGH indicates exposure to 1.75 Gy of γ-rays. RAR represents radio-adaptive response. LOW denotes exposure to 0.075 Gy of X-rays. b Quantitative analysis of caspase-3 protein levels. Amounts of caspase-3 were first normalized to β-actin, and then the ratio of each normalized value to its corresponding non-irradiated control value was calculated. Histograms represent the mean ± SD of normalized values obtained from three independent experiments. Open columns, non-irradiated thymocytes. Light gray columns, thymocytes exposed to 1.75 Gy of γ-rays. Dark gray columns, thymocytes treated with priming low dose X-irradiation (0.075 Gy) 6 h before a challenging high dose of γ-rays (1.75 Gy). Black columns, thymocytes exposed to 0.075 Gy of X-rays

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