Identification of oncogenic driver mutations by genome-wide CRISPR-Cas9 dropout screening

Kiessling, Michael K.; Schuierer, Sven; Stertz, Silke; Beibel, Martin; Bergling, Sebastian; Knehr, Judith; Carbone, Walter; de Vallière, Cheryl; Tchinda, Joelle; Bouwmeester, Tewis; Seuwen, Klaus; Rogler, Gerhard; Roma, Guglielmo

doi:10.1186/s12864-016-3042-2

BMC Genomics

Table 2 List of top 20 sgRNAs targeting kinases for CHP-212 cells

From: Identification of oncogenic driver mutations by genome-wide CRISPR-Cas9 dropout screening

Day 14			Day 21			Day 28
Kinase	sgRNA	Log2 fold change	Kinase	sgRNA	Log2 fold change	Kinase	sgRNA	Log2 fold change
TRIB2	HGLibA_58681	−5.96	STRADA	HGLibA_54860	−6.17	CLK2	HGLibA_10058	−5.49
STRADA	HGLibA_54860	−5.41	CDC7	HGLibA_08595	−6.09	CHEK1	HGLibA_09420	−5.37
BRD2	HGLibA_04772	−5.31	TRIB2	HGLibA_58681	−6.06	RIOK2	HGLibA_48597	−4.54
TEX14	HGLibA_56351	−5.14	PDK1	HGLibA_43184	−5.65	MAP2K1	HGLibA_35426	−3.98
MAP2K6	HGLibA_35443	−4.83	VRK1	HGLibA_61113	−5.64	GAK	HGLibA_18491	−3.78
CDC7	HGLibA_08595	−4.77	RIOK2	HGLibA_48597	−5.27	AATK	HGLibA_00089	−3.57
VRK1	HGLibA_61113	−4.75	BRD2	HGLibA_04772	−5.21	ACTR2	HGLibA_00655	−3.48
PDK1	HGLibA_43184	−4.59	TEX14	HGLibA_56351	−5.14	TP53RK	HGLibA_58364	−3.37
RPS6KB1	HGLibA_49465	−4.58	YES1	HGLibA_61950	−5.11	BRD2	HGLibA_04772	−2.97
ZAP70	HGLibA_62062	−4.57	GAK	HGLibA_18491	−5.02	PDK1	HGLibA_43184	−2.82
MAP2K1	HGLibA_35426	−4.52	PTK2	HGLibA_46667	−4.89	BRD2	HGLibA_04773	−2.81
RAF1	HGLibA_47416	−4.37	CHEK1	HGLibA_09418	−4.88	TRIB2	HGLibA_58681	−2.80
KIT	HGLibA_32303	−4.36	RIPK1	HGLibA_48603	−4.88	YES1	HGLibA_61950	−2.73
RIOK2	HGLibA_48597	−4.35	CHEK1	HGLibA_09420	−4.86	CDC7	HGLibA_08595	−2.72
SBK2	HGLibA_50091	−4.33	ZAP70	HGLibA_62062	−4.71	VRK1	HGLibA_61113	−2.67
GAK	HGLibA_18491	−4.32	RPS6KB1	HGLibA_49465	−4.70	STK38L	HGLibA_54791	−2.62
SYK	HGLibA_55237	−4.21	MAP2K1	HGLibA_35426	−4.60	SBK2	HGLibA_50091	−2.52
PTK6	HGLibA_46673	−4.11	MAP2K6	HGLibA_35443	−4.59	PKMYT1	HGLibA_44123	−2.46
RIOK1	HGLibA_48595	−3.98	KIT	HGLibA_32303	−4.57	NEK1	HGLibA_38926	−2.44
IGF1R	HGLibA_30081	−3.92	RPS6KA4	HGLibA_49455	−4.53	CDK11A	HGLibA_08726	−2.43

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ISSN: 1471-2164

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