Fig. 4

Ratio of mtDNA-II:mtDNA-I copy number in individual nematodes. Absolute copy numbers of each mtDNA circle were determined by qPCR using individual nematodes of different developmental stages, and the ratio of mtDNA-II to mtDNA-I copy number was calculated. The bold bars within the boxes indicate sample means, the edges of the boxes indicate first and third quantiles, and the whisker bars indicate minimum and maximum values (J2: n = 10, J3: n = 8 and J4: n = 8)