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Table 4 Non-digestive proteins identified by mass spectrometry

From: High throughput techniques to reveal the molecular physiology and evolution of digestion in spiders

Protein Digestive Fluid (%) Fasting (%) Fed (%) SPCS
Venom peptide isomerase (heavy chain) 0.53 N.I N.I None
 Venom allergen 5 0.44 N.I N.I Inc
 Venom allergen 5 0.22 N.I N.I Inc
 U24-ctenitoxin-Pn1a 1.82 1.6 0.38 17–18
 U24-ctenitoxin-Pn1a 0.11 N.I N.I 22–23
 U24-ctenitoxin-Pn1a 0.09 0.06 0.35 17–18
 U24-ctenitoxin-Pn1a 0.08 0.2 0.02 19–20
 U24-ctenitoxin-Pn1a 0.04 0.08 Id 17–18
 U24-ctenitoxin-Pn1a N.I 0.05 0.22 22–23
 U9-ctenitoxin-Pr1a N.I 0.009 Id Inc
Protease inhibitor U1-aranetoxin-Av1a N.I 0.41 0.57 None
 U3-aranetoxin-Ce1a N.I 0.06 I None
 L-cystatin 1.2 0.26 N.I 17–18
 Cystatin A2 N.I 0.7 1.16 None
 Serpin B3 0.06 0.07 0.03 Inc
 Serpin B6 N.I 0.03 N.I None
 Leukocyte elastase inhibitor 0.07 N.I N.I Inc
 Leukocyte elastase inhibitor 0.003 N.I N.I 21–22
 Alpha-1 inhibitor 3 0.04 0.008 0.002 27–28
 Peritrophin-1 N.I 0.16 0.74 Inc
 Peritrophin-48 0.008 0.04 N.I Inc
 Transferrin 0.2 0.09 0.63 Inc
 Soma ferritin 0.05 5.1 2.9 None
 Soma ferritin N.I 0.21 0.08 Inc
sum of leucine-rich repeat-containing proteins 5.9 a a a
  1. The values are the means of the percentages from the normalized spectral abundance factor to each sample. The data from midgut diverticula are from three distinct biological replicates and only proteins identified in at least two samples were used for quantitative analysis. Digestive juice NSAF is the sum of all different periods of feeding (fasting, 1, 3, 9, 25, 30 and 48 h
  2. Abbreviations: SPCS signal peptide cleavage site, Inc incomplete N-terminal sequence, N.I protein not identified, Id identified in only one replicate
  3. aprotein not searched in that sample or SPCS not analyzed