Fig. 2

Conservation of supercluster regions in several species. a Comparison of the fumagillin/pseurotin supercluster region among M. anisopliae, A. fumigatus and T. ophioglossoides. The backbone gene from fumagillin (green) is absent in M. anisopliae and T. ophioglossoides, but some accessory genes are present and intertwined with the well-conserved pseurotin BGC (red). These accessory genes appear to participate in metabolite biosynthesis; therefore, the final product of this cluster was speculated to be a pseurotin-related compound. Upstream of the pseurotin-related BGC, the fumitremorgin cluster (yellow) is present in A. fumigatus, but absent in M. anisopliae, and there is a putative tropolone/citrinin-related BGC at this location in T. ophioglossoides. The tropolone/citrinin-related BGC has orthologous sequences (MaPKS1) in M. anisopliae, although they are located in a different genomic region. The MaPKS14 (light-blue) BGC is located downstream the pseurotin-related BGC only in M. anisopliae. b Comparison of a putative supercluster region in M. anisopliae, A. fumigatus, A. niger, and P. ipomoeae. Three BGCs (helvolic acid, MaPKS18, and MaNRPS-PKS6) were assigned to this M. anisopliae region. The helvolic acid (pink) and MaPKS18 (purple) clusters appear to be co-regulated. Additionally, both are conserved in A. fumigatus and P. ipomoeae, although the BGCs are distantly located in chromosome 4 in A. fumigatus. * This locus was inverted to fit in the figure