Fig. 5

Tropolone/citrinin-related compound BGC (MaPKS1). a Phylogenetic analysis was performed using Maximum-likelihood and Bayesian methods, based on the tropolone/citrinin-related backbone gene and orthologous sequences in several fungi. Additionally, two PKS outgroup sequences were added: cichorine (Aspergillus nidulans FGSC A4) and mycophenolic acid (Penicillium brevicompactum). The orthologous sequences were classified according to fungal lifestyle trait, represented by different colors. The Bayesian tree is displayed, and branch support values (bootstrap proportions and Bayesian posterior probability) are associated with nodes. The Bayesian inference ran for 120,000 generations. Species in bold in (a) also have their domain organization shown with abbreviations (KS: Keto-synthase; AT: Acyltransferase; ACP: Acyl carrier protein; MT: Methyltransferase O- or C-; TD: Thioester reductase), and were used for the cluster conservation analysis presented in b. These clusters have characterized or partially characterized biosynthetic routes. b Some genes from M. anisopliae MaPKS1 BGC resembled the characterized stipitatic acid (tropolone) BGC from T. stipitatus and the citrinin BGC from M. purpureus. These conserved genes are involved in the first steps of the biosynthesis of their compound, as described in c. Note that the mrl1 gene of the citrinin biosynthetic pathway is absent in M. anisopliae. Additionally, the gene MANI_112402 resembles the ctnA citrinin regulator from M. purpureus (59 % identity), and putative transcription factors from C. posadasii, T. stipitatus, M. purpureus and M. pilosus as demonstrated in (b). Orthologous genes were assigned the same color; white boxes represent genes that are not predicted to be part of M. anisopliae cluster; and blue boxes represent the conserved transcription factor