Genome sequence of the filamentous soil fungus Chaetomium cochliodes reveals abundance of genes for heme enzymes from all peroxidase and catalase superfamilies

Table 5 Transcription analysis of 9 selected genes for peroxide catabolism in C. cochliodes recorded with RT-qPCR. Quantitative values representing relative changes of the transcription level were obtained by comparison of the expression of a particular gene in 30 min. induced vs. non induced samples. The constitutively expressed ITS1 region was used as internal standard for normalization

Changes in expression levels against non-induced control*
Analysed gene	Sample with 5 mM H₂O₂	Sample with 5 mM PAA
CcochhyBpox1	1.5 x	3.0 x
CcochhyBpox2	0.3 x	1.7 x
Ccochcyox1	0.3 x	2.3 x
Ccochlds	0.4 x	1.8 x
Ccochdyprx	3.3 x	18.5 x
Ccochhtp1	2.7 x	2.9 x
CcochkatA2	1.1 x	0.5 x
CcochkatB1	0.4 x	1.1 x
CcochkatB2	0.6 x	1.9 x

* Changes in the expression levels compared to the control sample (with the reference value of 1.0) were calculated as relative quantities due to the formula RQ = 2 ^{– ΔΔCq} where Cq is the quantification cycle of each RT-qPCR reaction. Presented are average values of triplicates for each listed gene and each inducer. Typical amplification plots and melting curves are presented in Additional file 4: Figure S2

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