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Fig. 12 | BMC Genomics

Fig. 12

From: Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)

Fig. 12

Validation of putative Soly WRKY Group III genes by virus induced gene silencing (VIGS). a Cotyledon agroinfiltration of pTRV vectors was conducted in the resistant cultivar ‘Zheza-301’ at the cotyledon stage. After three weeks, leaves with pTRV1 and pTRV2-SolyWRKY showed normal phenotype. Leaves of ‘Zheza-301’ with pTRV1 and pTRV2-PDS showed bleached areas. b The expression level of two SolyWRKY Group III genes in the VIGS treated plants one week after agroinfiltration. A-Tubulin (Solyc04g077020.2) was used as an internal reference. ‘A’ represented the ‘Zheza-301’ treated with pTRV1 and pTRV2 (negative control), ‘VIGS’ represented the ‘Zheza-301’ with pTRV1 and pTRV2-SolyWRKY. c The accumulation of TYLCV DNA in the SolyWRKY Group III genes silenced ‘Zheza-301’ plants. ‘A’ represented the ‘Zheza-301’ treated with pTRV1 and pTRV2 (negative control), ‘VIGS’ represented the ‘Zheza-301’ with pTRV1 and pTRV2-SolyWRKY

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