Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)

Huang, Ying; Li, Meng-Yao; Wu, Peng; Xu, Zhi-Sheng; Que, Feng; Wang, Feng; Xiong, Ai-Sheng

doi:10.1186/s12864-016-3123-2

Table 1 Primers used in the text

From: Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)

Gene	Direction	Sequence(5'-3')	Function
SolyWRKY41	Forward	ATGGAGAAAGTTAAAAGTATGGA	Full lengths clone
SolyWRKY41	Reverse	TTAAATGAAGAATTCTTCAATGTC	Full lengths clone
SolyWRKY54	Forward	ATGGATTGTGGATTCAATTATGAAT	Full lengths clone
SolyWRKY54	Reverse	TTATCTGAAAAAATCAGAGAAATTTG	Full lengths clone
SolyWRKY41	Forward	CACCATCACCATCACGCCATGATGGAGAAAGTTAAAAGTATGGA	Subcellular localization
SolyWRKY41	Reverse	CACTAGTACGTCGACCATGGCAATGAAGAATTCTTCAATGTC	Subcellular localization
SolyWRKY54	Forward	CACCATCACCATCACGCCATGATGGATTGTGGATTCAATTATGAAT	Subcellular localization
SolyWRKY54	Reverse	CACTAGTACGTCGACCATGGCTCTGAAAAAATCAGAGAAATTTG	Subcellular localization
SolyWRKY41	Forward	GTGAGTAAGGTTACCGAATTCGCAACACCAAACCATAACGCTGAA	VIGS vector conduction
SolyWRKY41	Reverse	GGGACATGCCCGGGCCTCGAGAGGAATTTGAAATCGAAGTCGGAGT	VIGS vector conduction
SolyWRKY54	Forward	GTGAGTAAGGTTACCGAATTCCCACAGAAACATAAACACAAGAAAC	VIGS vector conduction
SolyWRKY54	Reverse	GGGACATGCCCGGGCCTCGAGAGGAGAGACAAAAGATGGTGAATAA	VIGS vector conduction
SolyWRKY41	Forward	GCAACACCAAACCATAACGCTGAA	RT-qPCR
SolyWRKY41	Reverse	AGGAATTTGAAATCGAAGTCGGAGT	RT-qPCR
SolyWRKY42	Forward	AAGAAACTGTTCCTTTCACTCCACT	RT-qPCR
SolyWRKY42	Reverse	GAGCTGAACACAATACGATCCGATT	RT-qPCR
SolyWRKY53	Forward	CCACAACCAACATCGCCAGAGAA	RT-qPCR
SolyWRKY53	Reverse	ACGGTGAATAGCCGCTACCTATCA	RT-qPCR
SolyWRKY54	Forward	CCACAGAAACATAAACACAAGAAAC	RT-qPCR
SolyWRKY54	Reverse	AGGAGAGACAAAAGATGGTGAATAA	RT-qPCR
SolyWRKY80	Forward	TCACTGTCCAACTTCAAACTCTACT	RT-qPCR
SolyWRKY80	Reverse	ACGGTCTTGCTTCTCCTTCTTCT	RT-qPCR
SolyWRKY81	Forward	GCAATAGAAGGTTTAATTCGTGGTC	RT-qPCR
SolyWRKY81	Reverse	GTAGCGACGACGACATCAGA	RT-qPCR
SolyWRKY41	Forward	AGTGACATATAAAGGAAGGCACAGT	RT-qPCR for VIGS
SolyWRKY41	Reverse	CCCTTCTCCACCAAATGAGGAATT	RT-qPCR for VIGS
SolyWRKY54	Forward	GCAAGTGCAGAGGTCTGATGATGA	RT-qPCR for VIGS
SolyWRKY54	Reverse	TGTTGATGAAGTGTTGGCTGAGAAC	RT-qPCR for VIGS
TYLCV02	Forward	CGCCCGTCTCGAAGGTTC	RT-qPCR for VIGS
TYLCV02	Reverse	GCCATATACAATAACAAGGC	RT-qPCR for VIGS
TYLCV01	Forward	ATGTCGAAGCGACCAGGCGATATAAT	Detection for TYLCV DNA
TYLCV01	Reverse	TTAATTTGATATTGAATCATAGAAAT	Detection for TYLCV DNA
Tubulin	Forward	TGACGAAGTCAGGACAGGAA	Reference gene
Tubulin	Reverse	CTGCATCTTCTTTGCCACTG	Reference gene

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