Fig. 2

Detection of PHN using fluorescence microscopy. Plants were grown for 15 days on MS/2 sucrose-free medium and then transferred for 5 days on an identical medium supplemented with DMSO as a control (a, c, c’) or 200 μM PHN (b, d, d’, e, f). a-f panels show representative observations of samples under UV light. c’, d’ panels show bright field observations corresponding to the primary roots of C and D panels. Third leaf of control (a) and PHN-treated plants (b). Primary root of control (c) and PHN-treated plants (d). Bright spots corresponding to PHN observed in trichomes on the adaxial side of leaves (e). PHN aggregates on the epidermis on the abaxial side of leaves near stomatal guard cells, indicated by Circles (f)