Fig. 7

Effect of PHN on isolated mitochondria and thylakoids. The impact of PHN was analyzed using isolated spinach thylakoids (a, b) or pea seed mitochondria (c, d). For thylakoids, the light-driven oxygen production with ferricyanide as a photosystem I acceptor was measured using well-coupled thylakoids (a) or thylakoids uncoupled by by NH₄Cl (b). The arrows indicate the addition of the different compounds (final concentration) and the number below the line refers to the corresponding rate (μmol O₂.mg chlorophll⁻¹.min⁻¹). For pea seed mitochondria, PHN was added to isolated organelles oxidizing 5 mM succinate in state 4 (c) or state 3 (d). Arrows show the addition of compounds with their final concentration or amount in the case of ADP. Numbers under the lines indicate the rate of oxygen consumption in nmolO₂.min⁻¹.mg prot⁻¹