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Fig. 1 | BMC Genomics

Fig. 1

From: Distinct gene expression program dynamics during erythropoiesis from human induced pluripotent stem cells compared with adult and cord blood progenitors

Fig. 1

Gene expression during erythroid differentiation from adult stem cells in SEM-F. a PCA of differential gene expression in the triplicate AB FBS samples transforms the data into a series of uncorrelated variables made up from linear combinations and shows, in an unsupervised analysis, the progression of the differentiating erythroid cells through gene expression state-space. Genes reaching a minimum linear expression value of 100 in all replicates of at least one sample group were selected as differentially-expressed (DE) between any two stages during erythroid differentiation if they met the following criteria: p ≤ 0.01, fold change (FC) ≥ 2, B > 2.945 (Additional file 2: Table S1A). The union of all DE genes was used in the PCA. The Euclidean distances relating to this PCA are available in Additional file 19: Table S6. b Hierarchical clustering analysis of the differentially-expressed genes used in (A), clustering by gene only according to Euclidean distance. The colour bar on the left hand side denotes clusters of co-regulated genes. c Plot showing the number of differentially-expressed genes between consecutive populations in the adult FBS time-course data. d Genes described to be preferentially expressed in primary human erythroid cells [32] were examined in our current adult SEM-F dataset. Where these genes were expressed in our data, their expression pattern is shown as a hierarchical clustering, clustered by gene

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