Fig. 5

Transduction of hiPSC hematopoietic progenitors with lentivirus expressing c-KIT enhances erythroblast expansion. a Proliferation of hiPSC-erythroblasts transduced with either the GFP tagged lentiviral construct (GFP c-KIT) or with the control vector (GFP) was monitored after selection of GFP positive cells by FACS on day 2 of hiPSC erythroblast culture. Results from 2 independent experiments are shown in (i) and (ii). b Increased expression of c-KIT in erythroblasts grown from hiPSC progenitors transduced with the GFP c-KIT lentivral construct. Error bars are SDs of quadruplicate measurements by semi-quantitative PCR from cells taken on day 10 of culture showing a 1.5 fold increase that is comparable to the increase in cell expansion. c Protein expression of c-KIT at the surface of erythroblasts grown from hIPSC progenitors transduced with the GFP-c-KIT or control vector expressing GFP alone. GFP-c-KIT erythroblasts collected at the end of experiment (i) show a small increase in c-KIT expression where mean fluorescence intensity (MFI) is 47 compared with control MFI of 37 and the proportion of c-KIT positive cells is also modestly increased (highlighted in blue)