Fig. 1

Experimental design and sequencing read mapping. a Experimental design overview. b Genomic mapping of sequence reads. The proportion of reads that were unmapped (red), those mapping to single genomic positions (green), and those mapping to multiple locations (repeats, in blue) are illustrated. Reads mapping to single genomic positions are broken down into reads present as a unique copy, and those present in two or more identical copies (duplicates). Results shown are the mean of 5 replicates for each method, using 25 million reads per replicate. Error bars show the standard deviation from the mean