Fig. 2
From: A comparative study of ChIP-seq sequencing library preparation methods
Library complexity and H3K4me3 ChIP signals intensity. a Library complexity curves generated using Preseq. Yield of uniquely mapping reads based on down-sampled data of 25 million reads. PCR-free (three replicates) method from 100 ng input is shown in black. Red and blue lines represent the five replicates used in 1 ng and 0.1 ng input across six low-input methods. Actual and extrapolated values are shaded in white and grey, respectively. X-axis is represented in log2 scale. b Read depth in a 4 kb window centered on known TSS. Colours as in (a)