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Fig. 1 | BMC Genomics

Fig. 1

From: Cross-phenotype association tests uncover genes mediating nutrient response in Drosophila

Fig. 1

The ceramide synthase schlank governs body mass, starvation, and lifespan. a and b Trait association to schlank genotype in the DGRP. In a given panel, each point reports phenotype measurements (y-axis) from one DGRP strain homozygous for the schlank allele indicated on the x-axis. Each black horizontal bar reports the mean phenotype across all strains harboring the indicated allele. c-e Validation of schlank function. c Each trace reports survival of a strain harboring a schlank RNAi construct and a GAL4-GeneSwitch (GS) activator under the daughterless promoter, pre-treated with RU486 (+ drug, dotted lines) or vehicle (no drug, solid lines) for 10 days on the indicated diet and then switched to acute starvation media. d Each column reports body mass per fly of the indicated strain, with genotypes as in c (n = 2 populations of 15 flies per condition). e Each column reports whole body triglyceride levels per fly of the indicated strain, with genotypes as in c (n = 1 population of 15 flies per condition). For d and e top, middle, and bottom horizontal bars of a given vertical box denote the respective quartiles over batches and/or technical replicates, and the top and bottom short horizontal bars report minimum and maximum, respectively. AL, ad libitum diet; DR, dietary restriction. *, p < 0.05, **, p < 0.01, ***, p < 10-3, ****, p <10-7, *****, p < 10-15. Red asterisks denote significance of the effect of the genetic perturbation in animals on AL food, blue denotes significance in animals on DR food, and purple denotes significance of the interaction between diet and genetic perturbation. For strain details see Additional file 1: Table S6

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