Fig. 4

miR-27b enhances neurotransmission. a-c Whole-cell patch clamp recordings of mEPSCs in miR-27b KD and CT hippocampal neurons (DIV14). a Representative examples of individual mEPSC traces for both conditions. b Quantification of mEPSC frequency and amplitude in miR-27b CT (n = 25) and KD (n = 30). * p < 0.05, t-test. Red bars indicate the Mean and error bars represent SEM. Each symbol corresponds to data from a single neuron. c Cumulative distributions of mEPSC frequency and amplitude. d-f Immunostaining of VGLUT1 and VGAT in miR-27b KD and CT neurons. Scale bar: 10um. e,f Quantification of puncta density and intensity for (e) VGLUT1 (16 fields) and (f) VGAT (16 fields) from 3 experiments. *p < 0.05, t-test. g-i MEA recordings of miR-27b CT and KD cortical neurons (DIV15), or miR-27b KD neurons transduced with a Bmi1-targeting shRNA. g Bright-field (left) and mCherry fluorescence (right) images of a neuron network grown on an MEA chip. mCherry reports expression of miRZip-27b. Scale bar: 200um. h Quantification of burst frequency for the indicated conditions. Each symbol represents bursting activity of a single MEA culture dish. MEA dishes originate from three independent experiments. Red bars indicate the Mean (** p < 0.01, one-way ANOVA). i qPCR detection of Bmi1 transcripts in cortical neurons expressing miRZip-Scr, Bmi1-shRNA, miRZip-27b and miRZip-27b + Bmi1-shRNA (n = 7, * p < 0.05, ** p < 0.01, *** p < 0.001, one-way ANOVA). Error bars represent SEM