Fig. 3

Expression of TSG protein in mouse tissues and developing testis. TSG exists as a higher molecular weight form in the testis (a) In vitro translation using radioactively labelled methionine clearly shows two forms of TSG produced from the cDNA clone after autoradiography. Similar forms of TSG were detected using anti-TSG antibody in immunoblot analyses using the lysates of 293T cells transfected with HA-tagged TSG. (‡) stands for samples of in vitro translation using radioactive methionine. Empty pCDNA HA vector was used as a negative control (b) TSG expression during testis development (c)