Fig. 4
Functional analysis of the ScGluA1 gene encoding sugarcane beta-1,3-glucanase. a The infection results of Nicotiana tabacum Fusarium solani var. coeruleum and Botrytis cinerea after infiltration with the 35S::ScGluA1-containing Agrobacterium strain. Disease symptoms were assessed at 20 d post-inoculation. b The antimicrobial action of beta-1,3-glucanase (T0 generation of ScGluA1 transgenic N. benthamiana) on the hyphal growth of F. solani var. coeruleum. CK, the control of normal culture on F. solani var. coeruleum; 35S::ScGluA1, the antimicrobial action of beta-1,3-glucanase of the T0 generation of ScGluA1 transgenic N. benthamiana; 1 ~ 3, beta-1,3-glucanase from three different T0 generation plants of ScGluA1 transgenic N. benthamiana, respectively; 4 and 5, beta-1,3-glucanase from T0 generation of pCAMBIA 1301 transgenic and non-transgenic N. benthamiana, respectively; 6, 0.05 M sodium acetate buffer (pH 5.0). Red arrow indicates the antifungal effect. 2 d and 4 d, culture for 2 d and 4 d at 28 °C after crude enzyme fluid was added to the medium