Table 1 QRT-PCR primers used in verification of avian mRNAs results
From: Genome-wide profiling of chicken dendritic cell response to infectious bursal disease
Gene | Sence | Anti-sence | Product |
|---|---|---|---|
GAPDH | TGACCACTGTCCATGCCATC | CAGCAGCCTTCACTACCCTC | 273 bp |
β-ACTIN | tgtatgccaacacagtgctg | cctgcttgctgatccacatc | 204 bp |
MAPK Signaling Pathway | |||
EGF | gtactggtgcgatgctaagc | tgttttggccggtcttcttg | 185 bp |
PIK3CB | cacggtcgcatttggatcat | atccactgcccagatgtcaa | 202 bp |
MAP4K4 | cccattccttcagtgaccct | actggtgatcgggatgttgt | 208 bp |
FGF1 | ggcttgtaccaaagcagacc | gctgggctctcactcagtaa | 240 bp |
STAT1 | ttaacgaggagctggtggag | gaaaagactgtgcgttcggt | 233 bp |
SOD1 | taccggcttgtctgatggag | tcctccctttgcagtcacat | 172 bp |
PDGFB | tgaacccggcatgaatttcg | tctcgagacagggacacatg | 234 bp |
ELK4 | agagcccaaagagcaggatt | ggtcagcaagatgggagtct | 205 bp |
SPRED2 | atcctgatgacgtggactcc | accccgtttgttctcctcat | 230 bp |
TRAF2 | ccgttcctgctgattgagtg | cgcagccatcacaagtcaat | 190 bp |
NFATC2 | aactattgactgtgcgggga | ctcgatggggtttgatgctg | 172 bp |
MAP4K3 | tcagggtggctacttcttgg | tggtaaaggaggtggcactt | 163 bp |
EGFR | ttggagaagggagagcgttt | ccatgtcctcctcctccatc | 244 bp |
JAK-STAT signaling pathway | |||
PIK3CB | cacggtcgcatttggatcat | atccactgcccagatgtcaa | 202 bp |
SOCS3 | accactacatgcctcccac | cgttgacagtcttacggcag | 174 bp |
CREBBP | ttcccagcaactaggaccag | gccactttcctcttcctcct | 243 bp |
TPO | gggccaatctgcgacaatag | cacgccgcttaatctcatcc | 175 bp |
CCND3 | tttctggatgctggaggtgt | atgcagagcttctccacagt | 195 bp |
IL15 | gccggagagtcagaaaacac | agtgatttgcttctgtctttggt | 155 bp |
SPRED2 | agaacaaacggggtcaatgc | gcggcaagtaacagcacata | 231 bp |
OSMR | tcaagctgaagtgtggcaac | cgctccatctccagaaatgc | 161 bp |
IFNGR1 | tggagtcggcagaagatgtt | gaaaccactggacctgagga | 217 bp |