Fig. 2
From: Impact of RNA degradation on fusion detection by RNA-seq
Median coverage profile as a function of distance from 3′ end of mRNA and read decay across all protein-coding genes and genes involved in fusions for samples with different levels of degradation. a Median coverage per million reads (in log scale) as function of the distance from the 3′ end for UHR sample at different RIN values (8.6, 8.4, 7.6, 5.9, 4.9 and 3.9). Linear trend lines and 95 % confidence intervals are denoted in gray. b Median read decay rate per kilobase for all genes as a function of RIN for chemically degraded samples (UHR and U251 cell line). Notice that U251 had replicates. Linear trend line is shown in blue and 95 % confidence intervals are shown in gray. c Cumulative histogram of the distance of gene fusion breakpoints from the 3′ end for fusions in the COSMIC database. d Median read decay rate per kilobase for fusion related genes as function of RIN for a set of 20 fresh tissue specimens. Linear trend line is shown in blue and 95 % confidence intervals are shown in gray