Fig. 5
From: Analysis of gene expression in the midgut of Bombyx mori during the larval molting stage
Morphological changes and DNA synthesis in the molting midguts. (A1–F1) Morphology of midguts at feeding stages (A1, E1, and F1) and the molting stages (B1–D1) observed by microscopy. The tissue sections were stained using Mayer’s hematoxylin and eosin. Bubble-like structures appeared during the molting stage (indicated by arrowheads). No such structures were detected during the feeding stages. (A2–F2) Decreased DNA synthesis was noted in the midgut after entering the fourth larva-to-larva molting stage. DNA synthesis indicates midgut growth even during the molting stage. Developmental stages are indicated. MC: midgut contents. Bar: 50 μm