Comparison between two amplicon-based sequencing panels of different scales in the detection of somatic mutations associated with gastric cancer

Table 1 Targeted sequencing panels and the analyzed genes associated with gastric cancer

Panel name	Targets size	No. of Amplicons	No. of genes	Covered rate	Gene list
Selective hotspot Panel	38.01 kb	376	20	99.99 %	APC, ARID1A, BCOR, CDH1, CTNNB1, EGFR, ERBB2, ERBB3, FGFR2, JAK2, KRAS, MET, NRAS, PIK3CA, PTEN, RASA1, RHOA, RNF43, SMAD4, TP53*
Comprehensive Panel	351.05 kb	3515	58	96.86 %	ABCA10, ACVR2A, AKAP13, *APC* , ARHGAP5, *ARID1A* , *BCOR* , BNC2, CD274, *CDH1* , CNGA4, CTNNA1, CTNNA2, CTNNB1* , DLC1, DNAH7, *EGFR* , EIF2C4, ELF3, *ERBB2* , *ERBB3* , EYA4, FAM46D, FAT4, FGFR1, *FGFR2* , GLI3, *JAK2* , KIF2B, KMT2A, KMT2C, KRAS* , LDOC1, MACF1, *MET* , MUC6, NRAS* , PCDH9, PDCD1LG2, *PIK3CA* , PIK3R1, PKHD1, PLB1, *PTEN* , PTPRC, *RASA1* , RGNEF, RHOA* , *RNF43* , SMAD2, *SMAD4* , SOHLH2, SYNE1, TGFBR2, TMPRSS2, *TP53* , VEGFA, ZIC4

Genes shown in bold font represent the 20 identified by the Selective hotspot Panel
*Genes targeting hotspot regions

ISSN: 1471-2164