Table 2 Coverage depth of the data from the two panels
Selective hotspot Panel | Comprehensive Cancer Panel | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Case | Sample | Mapped Reads | On Target | Mean Depth | Uniformity | Mapped Reads | On Target | Mean Depth | Uniformity |
Case 1 | Buffy coat | 484124 | 98.0 % | 1402 | 94.0 % | 1097404 | 96.4 % | 307 | 96.0 % |
Case 2 | Buffy coat | 426768 | 98.1 % | 1233 | 94.0 % | 1267404 | 96.3 % | 355 | 96.1 % |
Case 3 | Buffy coat | 518868 | 98.2 % | 1507 | 93.8 % | 810553 | 96.3 % | 221 | 95.0 % |
Case 4 | Buffy coat | 542769 | 98.4 % | 1576 | 93.9 % | 379011 | 96.1 % | 106 | 94.5 % |
Case 5 | Buffy coat | 624920 | 97.9 % | 1796 | 94.3 % | 11170406 | 96.8 % | 3112 | 95.1 % |
Case 6 | Buffy coat | 164634 | 98.9 % | 477 | 93.5 % | 2069779 | 97.9 % | 611 | 90.6 % |
Case 7 | Buffy coat | 126183 | 99.0 % | 363 | 91.0 % | 2256973 | 97.8 % | 666 | 91.0 % |
Case 8 | Buffy coat | 108641 | 99.1 % | 315 | 90.5 % | 2027465 | 97.7 % | 601 | 92.0 % |
Case 9 | Buffy coat | 166684 | 99.0 % | 487 | 94.7 % | 1857285 | 97.8 % | 551 | 92.2 % |
Case 10 | Buffy coat | 107898 | 99.0 % | 314 | 90.7 % | 2013379 | 98.0 % | 593 | 89.0 % |
Case 11 | Buffy coat | 79551 | 99.2 % | 231 | 87.1 % | 2106246 | 98.1 % | 624 | 88.7 % |
Case 12 | Buffy coat | 67486 | 99.2 % | 197 | 88.3 % | 1859173 | 98.3 % | 542 | 82.5 % |
Case 13 | Buffy coat | 60485 | 99.2 % | 175 | 91.2 % | 1918204 | 98.3 % | 548 | 78.6 % |
Case 14 | Buffy coat | 260250 | 98.8 % | 759 | 92.4 % | 1295372 | 96.7 % | 360 | 94.9 % |
Case 15 | Buffy coat | 235410 | 98.7 % | 680 | 94.2 % | 1053705 | 96.9 % | 293 | 94.6 % |
Case 16 | Buffy coat | 246227 | 99.0 % | 715 | 93.7 % | 845571 | 97.0 % | 234 | 94.7 % |
Case 17 | Buffy coat | 268465 | 98.8 % | 779 | 94.4 % | 1223358 | 96.8 % | 341 | 95.2 % |
Case 18 | Buffy coat | 280097 | 99.1 % | 811 | 93.9 % | 3130126 | 97.0 % | 902 | 93.6 % |
Case 19 | Buffy coat | 256281 | 98.9 % | 744 | 94.5 % | 3423132 | 97.0 % | 987 | 94.4 % |
Case 20 | Buffy coat | 207402 | 98.9 % | 598 | 92.4 % | 2913580 | 97.0 % | 830 | 93.6 % |
Mean ± SD | 261657 ± 170658 | 98.7 ± 0.42 % | 758 ± 493 | 92.6 ± 2.1 % | 2235906 ± 2247475 | 97.2 ± 0.71 % | 639 ± 627 | 92.1 ± 4.6 % | |
Case 1 | Tumor | 403252 | 98.5 % | 1143 | 87.8 % | 2025838 | 97.7 % | 522 | 71.0 % |
Case 2 | Tumor | 426999 | 98.4 % | 1222 | 91.6 % | 999656 | 96.9 % | 276 | 94.1 % |
Case 3 | Tumor | 524706 | 98.5 % | 1502 | 91.8 % | 1032722 | 96.3 % | 281 | 94.6 % |
Case 4 | Tumor | 467625 | 98.0 % | 1337 | 91.0 % | 789820 | 95.8 % | 210 | 95.2 % |
Case 5 | Tumor | 412941 | 98.5 % | 1186 | 90.7 % | 829933 | 96.0 % | 223 | 94.9 % |
Case 6 | Tumor | 72752 | 99.3 % | 207 | 84.8 % | 1949114 | 98.6 % | 577 | 83.2 % |
Case 7 | Tumor | 119809 | 99.3 % | 340 | 85.8 % | 2511041 | 97.6 % | 718 | 91.2 % |
Case 8 | Tumor | 85768 | 99.2 % | 246 | 88.2 % | 1521154 | 98.6 % | 437 | 82.4 % |
Case 9 | Tumor | 182739 | 99.2 % | 523 | 89.0 % | 2048473 | 98.2 % | 583 | 88.3 % |
Case 10 | Tumor | 110281 | 98.0 % | 311 | 88.5 % | 2702014 | 97.3 % | 762 | 87.2 % |
Case 11 | Tumor | 96338 | 99.2 % | 273 | 89.8 % | 2668972 | 98.0 % | 750 | 86.1 % |
Case 12 | Tumor | 106265 | 99.2 % | 303 | 87.7 % | 2382665 | 97.8 % | 673 | 89.0 % |
Case 13 | Tumor site1 | 87099 | 98.8 % | 250 | 92.4 % | 2861114 | 97.5 % | 819 | 91.1 % |
Case 13 | Tumor site2 | 138279 | 99.3 % | 395 | 88.9 % | 2705723 | 97.9 % | 777 | 91.5 % |
Case 14 | Tumor | 230616 | 98.9 % | 662 | 92.6 % | 781688 | 96.5 % | 211 | 95.1 % |
Case 15 | Tumor | 262428 | 99.1 % | 742 | 65.8 % | 631147 | 96.6 % | 171 | 95.2 % |
Case 16 | Tumor | 179627 | 99.1 % | 512 | 87.8 % | 3189103 | 95.9 % | 929 | 95.8 % |
Case 17 | Tumor | 153577 | 99.0 % | 434 | 85.7 % | 539462 | 96.5 % | 145 | 95.0 % |
Case 18 | Tumor | 145284 | 98.4 % | 411 | 94.7 % | 2514172 | 96.4 % | 697 | 91.0 % |
Case 19 | Tumor | 184785 | 97.6 % | 520 | 94.0 % | 2333968 | 95.0 % | 637 | 91.4 % |
Case 20 | Tumor | 105210 | 97.8 % | 294 | 95.0 % | 1663278 | 96.6 % | 436 | 80.3 % |
Mean ± SD | 214113 ± 143314 | 98.7 ± 0.54 % | 610 ± 411 | 88.7 ± 6.0 % | 1841955 ± 850360 | 97.0 ± 1.0 % | 516 ± 247 | 89.7 ± 6.3 % | |