Fig. 2

Three genomic segments require the SOX10 consensus sequence for activity in Schwann cells. a The seven active regions from Fig. 1 were tested in forward and reverse orientation in rat Schwann (S16) cells. Luciferase data are expressed relative to a control vector without a genomic segment (‘Empty’). Error bars indicate standard deviations and arrows and lines indicate genomic segments that are active in both orientations. b Luciferase reporter gene constructs containing either the wild-type sequence (WT) or the sequence lacking the SOX10 consensus sequence(s) (ΔSOX10) were transfected into S16 cells and tested in luciferase assays. The luciferase activity associated with each ΔSOX10 construct is expressed relative to the respective wild-type construct. Error bars indicate standard deviations and arrows and lines indicate genomic segments with a required SOX10 consensus sequence