Fig. 5

SOX10 is necessary and sufficient for SOX6 expression. a RT-PCR was performed to detect the expression of Sox6 transcripts harboring exon 1G using cDNA isolated from S16 cells, MN1 cells, or MN1 cells transfected with no expression construct (mock) or a construct to express wild-type or dominant-negative (E189X) SOX10. Base pair (bp) ladders are indicated on the left. RT-PCR for β-actin and samples including no cDNA (‘Blank’) were employed as positive and negative controls, respectively. Please note that while the same primers were used for each reaction, the rat (S16) PCR product was 402 base pairs and the mouse (MN1) PCR product was 349 bp; the rat genome harbors a 53 base pair rat-specific insertion, which we confirmed via DNA sequence analysis. b Rat Schwann (S16) cells were treated with a control siRNA (left side) or a siRNA targeted against Sox10 (right side). Quantitative RT-PCR was used to measure expression levels of total Sox6 (green bars) or Sox6 exon 1G-containing (purple bars) transcripts. Error bars indicate standard deviations