Fig. 5

Comparative RNA-seq and qPCR calculated expression of PRV responsive genes. For each select PRV responsive unigene, DESeq2 normalized counts (left) are presented alongside relative quantities assessed by qPCR from the same samples used to prepare RNA-seq libraries (middle) or with additional samples not incorporated into RNA-seq analysis where available (right) normalized to two stable reference genes. Samples not included in RNA-seq but with identical condition-specific parameters are indicated (black border). For groups which received mock PRV injections, samples which received L-15 media injections (open circles) are distinguished from those which received blood homogenate injections (filled circles). Significant differential expression between experimental groups are indicated at p < 0.05 (*), 0.01 (**) and 0.001 (***) by t-test or at p < 0.05 by ANOVA with post hoc Tukey test where appropriate. Group 1: mock; Group 2: PRV; Group 3: mock; Group 4: IHNV exposed qPCR-; Group 5: IHNV exposed qPCR+; Group 6: PRV; Group 7: PRV/IHNV exposed qPCR-; Group 8: PRV/IHNV exposed qPCR+ (Table 1)