Fig. 1

Characterization of small RNAs associated with VcAGO3. a Domain structure of V. carteri VcAGO3. VcAGO3 contains the canonical domains PAZ and Piwi and a domain of unknown function (DUF1785). Domain borders are indicated by the position of amino acid residues. b Western blot showing the expression and successful immunoprecipitation (IP) of over expressed, myc-tagged VcAGO3 and myc-tagged CrGFP (GFP, codon optimized for C. reinhardtii [49]). T = total lysate; IP = immunoprecipitation. c Size distribution of all small RNA reads associated with VcAGO3 measured by deep sequencing (after adapter trimming and quality filtering, see methods), nt = nucleotide. d Nucleotide frequencies of all small RNAs associated with VcAGO3 (e) Pie chart showing the categorization of all mapped reads. RE, repetitive elements. f Pie chart showing the distribution of all mapped, assigned reads. RE, repetitive elements. g Nucleotide frequencies of all miRNA reads