Skip to main content

Table 3 Streptobacillus moniliformis specific Variable Number of Tandem Repeat (VNTR) primer sequences used in this study

From: Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis

Primer ID VNTR positiona Repeat size in nt (identity in %) Sequence (5–3) PCR product size (bp)
VNTR_Sm1 1576120 - 1576145 3 (100) TCA TTT ACT CAC CCT AGT AGT GGT 210
CCA GTT GAA TAT AAG CTT GCT ATG G
VNTR_Sm2 1182890 - 1182907 6 (100) TGG AAC TGT TTG TTG AGT ATT TCC A 298
AGG GAC AGA TGT TCA ATT TGT GTA
VNTR_Sm3 284997 - 285268 36 (91) TAC GCT GTA GGG TTG AAC GG 830
ACA GTT TGA GCA CGT CTT AAT CC
  1. Primers were designed with Geneious (v. 8.1.3; Biomatters, Auckland, NZ) [43] and to be complementary to VNTR flanking regions that were conserved among genomes; aaccording to the S. moniliformis DSM 12112T genome (CP001779.1)