Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis

Table 3 Streptobacillus moniliformis specific Variable Number of Tandem Repeat (VNTR) primer sequences used in this study

Primer ID	VNTR position^a	Repeat size in nt (identity in %)	Sequence (5–3)	PCR product size (bp)
VNTR_Sm1	1576120 - 1576145	3 (100)	TCA TTT ACT CAC CCT AGT AGT GGT	210
VNTR_Sm1	1576120 - 1576145	3 (100)	CCA GTT GAA TAT AAG CTT GCT ATG G	210
VNTR_Sm2	1182890 - 1182907	6 (100)	TGG AAC TGT TTG TTG AGT ATT TCC A	298
VNTR_Sm2	1182890 - 1182907	6 (100)	AGG GAC AGA TGT TCA ATT TGT GTA	298
VNTR_Sm3	284997 - 285268	36 (91)	TAC GCT GTA GGG TTG AAC GG	830
VNTR_Sm3	284997 - 285268	36 (91)	ACA GTT TGA GCA CGT CTT AAT CC	830

Primers were designed with Geneious (v. 8.1.3; Biomatters, Auckland, NZ) [43] and to be complementary to VNTR flanking regions that were conserved among genomes; ^aaccording to the S. moniliformis DSM 12112^T genome (CP001779.1)

ISSN: 1471-2164