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Table 3 Transcripts with functional annotations related to environmental stress responses exhibiting significant (P adj < 0.05) and biologically relevant (log2 fold-change relative to controls > 1 or < -1) changes in abundance in either treatment group

From: De novo assembly and analysis of changes in the protein-coding transcriptome of the freshwater shrimp Paratya australiensis (Decapoda: Atyidae) in response to acid sulfate drainage water

Category and transcript ID

Assigned description

Gene Ontology terms (or conserved domains)

Log2FC 50 % ADW

Padj  50 % ADW

Log2FC 100 % ADW

Padj 100 % ADW

Oxidative stress responses / oxidoreductase activity

  TR14472|c0_g2_i1|m.3443

chorion peroxidase

F:peroxidase activity; F:heme binding; P:response to oxidative stress; P:peroxidase reaction

2.29

0.011

1.09

0.770

  TR84113|c0_g1_i2|m.82050

aldehyde oxidase

F:electron carrier activity; F:flavin adenine dinucleotide binding; F:iron ion binding; F:2 iron, 2 sulfur cluster binding; F:oxidoreductase activity, acting on CH-OH group of donors; P:electron transport

1.02

0.0006068

1.16

5.41E-05

  TR21531|c3_g1_i2|m.10028

peroxisomal n -acetyl-spermine spermidine oxidase-like

F:oxidoreductase activity

3.00

4.18E-05

2.84

0.00026

  TR29581|c1_g2_i2|m.19009

spermatogenesis-associated protein 18-like protein

P:cellular response to stress; C:mitochondrial outer membrane

1.06

0.00024

0.86

0.011

  TR39455|c0_g1_i3|m.30019

failed axon connections homolog

Thioredoxin-like domain PF13410 (PFAM)

4.46

5.82E-15

4.10

3.02E-12

  TR41138|c2_g1_i2|m.32139

glutathione peroxidase 3

P:response to oxidative stress; F:glutathione peroxidase activity; P:glutathione metabolic process; P:peroxidase reaction

−1.17

0.012

−0.571

0.74

  TR41138|c2_g1_i1|m.32138

glutathione peroxidase 6

P:response to lipid hydroperoxide; C:extracellular space; P:hydrogen peroxide catabolic process; C:extracellular vesicular exosome; F:selenium binding; F:transcription factor binding; F:glutathione peroxidase activity; P:protein homotetramerization; C:transcription factor complex; P:glutathione metabolic process; P:peroxidase reaction

−1.04

0.024

−0.476

0.86

Metal binding activity

  TR70803|c0_g1_i1|m.66800

metal transporter cnnm4

F:adenyl nucleotide binding

−0.38

0.52

−0.88

0.022

Ion channel activity

  TR82036|c1_g1_i1|m.79405

epithelial chloride channel partial

-

1.04

0.027

1.05

0.043

  TR64328|c0_g1_i1|m.60446

na + k + -atpase alpha subunit

F:metal ion binding; P:ATP biosynthetic process; F:ATP binding; F:sodium:potassium-exchanging ATPase activity; C:sodium:potassium-exchanging ATPase complex

−1.38

0.21

−1.98

0.045

  TR61105|c5_g1_i2|m.55789

na + k + -atpase alpha subunit

F:metal ion binding; P:ATP biosynthetic process; F:ATP binding; F:sodium:potassium-exchanging ATPase activity; C:sodium:potassium-exchanging ATPase complex

−0.46

0.291

−0.73

0.043

Cell death / apoptosis

  TR80082|c0_g1_i2|m.75465

programmed cell death protein 2

F:binding; P:programmed cell death; C:nucleus; C:cytoplasm

1.31

0.0814986

1.62

0.027945

  1. Abbreviations: Log 2 FC logarithm to base 2 of fold-changes in transcript abundance relative to controls, ADW acid drainage water, P adj , Benjamini-Hochberg corrected P value