Fig. 4
RNAseq coverage is low upstream of the rTSSs and a ~10 nt shift illustrates the loss of 5’-end information in a standard RNAseq protocol. The RNAseq coverage, from Illumina TruSeq stranded RNA sequencing, was determined for each nucleotide in a region from 50 nt upstream (-50) to 250 nt downstream (+250) of each of the 2018 rTSSs identified for S. aureus. The median RNAseq coverage from the two biological replicates is shown in red and blue. The dotted lines correspond to the 25th and 75th percentiles, respectively. A grey dotted line indicates the superimposed positions of the 2018 rTSSs, and a black bracket indicates the ~10 nt shift between coverage in the TruSeq protocol and the exact 5’-mapping of the TSS-EMOTE. The traces of RNAseq coverage for each of the 2018 rTSSs is shown in Additional file 2: Figure S1