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Fig. 6 | BMC Genomics

Fig. 6

From: Transcription factor ZNF25 is associated with osteoblast differentiation of human skeletal stem cells

Fig. 6

ZNF25 siRNA knockdown increases the expression of MMP1, LGR5, RANBP3L and other osteoblastic differentiation genes. Volcano plot representing the 512 genes most differentially expressed between siZNF25 knockdown and control hMSCs at day 14 post-OB induction. The siZNF25/control fold change for each gene is represented on the x axis, with the corresponding p value represented on the y axis (expressed as –log(p value)). Genes which have the Gene Ontology (GO) annotation ‘extracellular matrix organisation’ are coloured in red. Genes with GO annotation ‘skeletal system development’ are coloured in green and those with GO annotation ‘regulation of ossification’ are coloured in blue. Genes in these three categories are listed on the right. This figure represents ‘extracellular matrix organisation’, ‘skeletal system development’ and ‘regulation of ossification’ annotation categories only, as genes with the annotations ‘ossification’ and ‘bone development’ are all contained within the other 3 categories. There were four very highly upregulated genes (labelled on the plot). These are matrix metallopeptidase 1 (MMP1, FC = 23.00, pval = 6.09 × 10–6), leucine-rich repeat containing G protein-coupled receptor 5 (LGR5, FC = 18.59, pval = 5.77 × 10–7), RAN binding protein 3-like (RANBP3L, FC = 18.08, pval = 1.81 × 10–9) and unannotated transcript cluster, 17118303 (FC = 26.05, pval = 7.49 × 10–6). On further investigation it was found that this latter transcript cluster maps to chromosomal location: chr7:94058513–94060553, which corresponds to exon 52 of the COL1A2 gene. LGR5 and RANBP3L have very similar expression values and so the dots on the volcano plot for each gene are overlapping

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