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Fig. 7 | BMC Genomics

Fig. 7

From: Arabidopsis thaliana population analysis reveals high plasticity of the genomic region spanning MSH2, AT3G18530 and AT3G18535 genes and provides evidence for NAHR-driven recurrent CNV events occurring in this location

Fig. 7

LCRs bordering AT3G18530 and AT3G18535 genes and their role in mediating NAHR-based gene copy number changes. a - Hypothesized model of nonhomologous pairing between the left (Chr3:6372413..6373650, red) and right (Chr3:6377368..6378605, blue) LCR, leading to deletion (in case of intrachromatidial NAHR) or to deletion and reciprocal duplication (in case of interchromatidial/interchromosomal NAHR) of the flanked genomic DNA. The actual site of strand exchange and the length of the conversion track determine the sequence of the repeat reconstituted after recombination: identical to one of the original LCRs or chimeric. Pink arrows show the estimate localizations of primers used for amplification of the breakpoint region in accessions with “del-2” genotype. Yellow arrows show the estimate localization of primers used for amplification of the breakpoint region in accessions with “dupl-2” genotype. RSE – region of strand exchange; b - Representative gel image of genomic DNA fragments amplified in accessions with “del-2” genotype and in Col-0 (“basic” genotype). The expected length of DNA amplicon is 3,421 bp (8,376 bp in case of Col-0); c - Gel image of genomic DNA fragments amplified in accessions with “dupl-2” genotype and in Col-0 (“basic” genotype). The expected length of DNA amplicon is 3,404 bp (no product in case of Col-0)

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BMC Genomics

ISSN: 1471-2164

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