Table 1 Whole-genome capture of B. microti from mixed DNA templates of tick and human samples
Sample | Site | State | Collection year | Source | B. microti genome copiesa | Capture efficiency | Reference covered (%) | Mean Chr coverage | Mean apicoplast coverage |
|---|---|---|---|---|---|---|---|---|---|
CT14-14 | Mansfield | CT | 2014 | human blood | 685,844 | 98.45 | 98.9 | 1937.3 | 1322.2 |
N11-23 | Nantucket | MA | 2011 | human blood | 164,819 | 97.75 | 98.8 | 1808.0 | 2168.8 |
N11-46 | Nantucket | MA | 2011 | human blood | 81,207 | 97.49 | 98.8 | 1402.1 | 1291.3 |
ME14-04 | Portland | ME | 2014 | human blood | 389,507 | 97.15 | 98.8 | 1071.0 | 714.8 |
ME13-07 | Portland | ME | 2013 | human blood | 136,878 | 96.14 | 98.8 | 480.0 | 202.0 |
N14-18 | Nantucket | MA | 2014 | human blood | 136,892 | 95.92 | 98.8 | 758.5 | 444.8 |
CT14-17 | Mansfield | CT | 2014 | human blood | 118,180 | 95.78 | 98.7 | 216.3 | 134.6 |
NY-1509 | Mashomack Preserve | NY | 2011 | nymphal tick | 22,259 | 95.53 | 98.7 | 176.5 | 598.8 |
CT-1807 | Barn Island WMA | CT | 2013 | nymphal tick | NA | 70.87 | 98.7 | 195.5 | 889.0 |
MA-2296 | Quashnet River State Park | MA | 2013 | nymphal tick | 9931 | 69.41 | 98.7 | 312.4 | 1003.2 |
N-1725 | Squam Swamp | MA | 2013 | nymphal tick | 6919 | 91.76 | 98.6 | 246.4 | 1135.4 |
N14-004 | Nantucket | MA | 2014 | human blood | 67,064 | 88.46 | 98.5 | 112.6 | 133.9 |
N14-8 | Nantucket | MA | 2014 | human blood | 1700 | 84.53 | 98.4 | 74.7 | 69.8 |
NY-1477 | Connetquot State Park | NY | 2011 | nymphal tick | NA | 80.66 | 98.4 | 63.6 | 105.1 |
WI-205 | Black River State Forest | WI | 2011 | nymphal tick | NA | 45.91 | 97.9 | 43.8 | 107.7 |
N-1729 | Squam Swamp | MA | 2013 | nymphal tick | NA | 48.11 | 97.0 | 33.8 | 173.1 |
NY-1468 | Connetquot State Park | NY | 2011 | nymphal tick | 2314 | 16.26 | 96.4 | 26.5 | 69.0 |
NY-2455 | James Baird State Park | NY | 2011 | nymphal tick | NA | 66.68 | 95.8 | 26.4 | 58.1 |
MA-1689 | Manuel F. Correllus State Forest | MA | 2013 | nymphal tick | 2256 | 86.12 | 94.8 | 34.3 | 248.6 |
MA-2473 | Nickerson State Park | MA | 2013 | nymphal tick | 4904 | 84.35 | 93.7 | 22.5 | 23.6 |
NH-2440 | Great Bay National Wildlife Refuge | NH | 2011 | nymphal tick | 7454 | 58.49 | 93.6 | 35.4 | 67.3 |
NY-2464 | James Baird State Park | NY | 2011 | nymphal tick | NA | 43.7 | 92.3 | 20.5 | 44.0 |
N11-15 | Nantucket | MA | 2011 | human blood | 2218 | 65.07 | 90.0 | 18.9 | 12.8 |
CT14-1 | Woodbridge | CT | 2014 | human blood | 8,025 | 85.84 | 81.9 | 15.4 | 11.0 |
WI-197 | Black River State Forest | WI | 2011 | nymphal tick | NA | 46.74 | 74.0 | 14.2 | 7.9 |
NY-2539 | Hither Hills State Park | NY | 2013 | nymphal tick | NA | 3.25 | 8.6 | 33.0 | 23.2 |
NY-2458 | James Baird State Park | NY | 2013 | nymphal tick | 3191 | 81.95 | 4.5 | 4.2 | 4.3 |
MA-1670 | Manuel F. Correllus State Forest | MA | 2013 | nymphal tick | NA | 20.06 | 2.2 | 1.2 | 63.5 |
CT-2493 | Rocky Neck SP | CT | 2013 | nymphal tick | NA | 5.97 | 2.1 | 0.6 | 8.0 |
NY-2387 | Ward Pound Ridge | NY | 2013 | nymphal tick | 268 | 17.22 | 1.9 | 0.4 | 7.0 |
NY-1524 | Montauk Point State Park | NY | 2013 | nymphal tick | 59 | 8.48 | 1.9 | 0.4 | 9.3 |
RI-2584 | Block Island | RI | 2013 | nymphal tick | NA | 20.69 | 1.7 | 3.1 | 21.7 |
ME-2737 | Cape Elizabeth | ME | 2013 | nymphal tick | 324 | 10.63 | 1.6 | 0.3 | 6.0 |
MA-2678 | Nickerson State Park | MA | 2013 | nymphal tick | NA | 2.68 | 1.5 | 0.3 | 8.0 |
CT-1824 | Barn Island WMA | CT | 2013 | nymphal tick | 224 | 38.53 | 1.1 | 2.8 | 8.8 |
NJ-2719 | Naval Weapons Station Earle | NJ | NA | nymphal tick | 118 | 31.21 | 1.1 | 2.1 | 17.1 |
NY-1587 | Mashomack Preserve | NY | 2013 | nymphal tick | NA | 22.26 | 0.8 | 2.6 | 8.9 |
RI-2589 | Robins Hollow | RI | 2013 | nymphal tick | 105 | 5.99 | 0.6 | 0.2 | 9.2 |
CT-2413 | 50-Foot Cliff | CT | 2013 | nymphal tick | 200 | 14.05 | 0.4 | 0.0 | 2.0 |
WI-A406 | Jackson County | WI | NA | female adult tick | NA | 39.53 | 0.4 | 0.6 | 0.4 |
CT-2498 | Rocky Neck SP | CT | 2013 | nymphal tick | NA | 19.22 | 0.2 | 0.0 | 0.4 |
NY-2600 | Hither Hills State Park | NY | 2013 | nymphal tick | 15 | 0.31 | 0.2 | 0.3 | 6.4 |
WI-A118 | Spooner Veterinary Clinic, Washburn Co. | WI | NA | female adult tick | NA | 3.74 | 0.1 | 0.2 | 2.0 |
WI-A424 | Jackson County | WI | NA | female adult tick | NA | 15.44 | 0.1 | 0.0 | 0.2 |