Fig. 1

Principal component analysis of the mouse salivary glands at different developmental time points. a Experimental scheme. We isolated total RNA from whole salivary glands ranging from early embryo to adult, and performed RNA-seq. Utilizing these datasets, we defined and annotated the salivary gland transcriptional landscape by using various Gene Ontology (GO) annotation analyses (BiNGO GO, REVIGO GO) and pathway analyses (PANTHER/REACTOME/KEGG). b Proportion of variance in each principle component. PC1, PC2 and PC3 represent ~90% of variance in the data. c Projection plots show the PCA coordinates for each stage, which are indicated by different colors. The data indicates that the inherent variations in gene expression between biological samples can distinguish the developing salivary gland in a time dependent manner