Fig. 2

Three distinct Sp185/333 gene amplicon size patterns are detected in large insert BAC clones. Amplicon peak height and area indicate three major amplicon size patterns from fragment analysis, which are designated as Cluster 1 (a), Cluster 2 (b) and Cluster 3 (c). Amplicon size patterns identified less often (1 or 2 clones of 27 analyzed; see Additional file 3: Figure S2A) and that partially match amplicons in either Cluster 1 or 2 are designated as Cluster 1′, Cluster 1″ (a) or Cluster 2′ (b). Fragment length analysis of each cluster was carried out for each of the corresponding BACs (listed below) using primers F6 and R9 (see Additional file 1: Table S1; Additional file 2: Figure S1B). Means and standard error were calculated when multiple BACs were used for analysis of Clusters 1 and 2. Cluster 1 BACs are 10A2, 10B1, 10C6, 4074 J14, 4079E24, 61 M13. Cluster 2 BACs are 4C3, 4007 J10, 4024 N22, 4029 F3, 4091I3, 10 K9, 64C18, 4011G4. Cluster 3 BACs are 10C18, 10H9, 10H10, 10 M18, 3090I9, 3104 N4, 3104P4, 4028 F7, 4067A10. Cluster 1′ BACs are 3033E12, 4093A10. Cluster 1″ BAC is 4093A10. The Cluster 2′ BAC is 409O3