Fig. 6

Representative images of lipid droplets in COS-1 cells transfected with (a and b) AcGFP1-CIDEC (R46) and (c and d) AcGFP1-CIDEC (S46). a and c present cells cultured in media without oleic acid (OA) and b and d present cells cultured in media with OA. Images of nuclei labeled with DAPI, neutral lipid labeled with Nile red and location of AcGFP1 were merged. e Quantification of lipid specifically associated with GFP in cells. Using ImageJ software, the ratio of Nile red/GFP signal was calculated from 34 lipid droplet clusters each for R46 and S46 without OA and 47 lipid droplet clusters for R46 and 46 lipid droplet clusters for S46 with OA. Data are presented as means ± SEM. *P < 0.05