Marker development using SLAF-seq and whole-genome shotgun strategy to fine-map the semi-dwarf gene ari-e in barley

Table 5 PCR primers, product size and their physical position designed from whole-genome shotgun strategy

Marker name	Forward sequence	Reverse sequence	Size	POPSEQ Phyiscal Position
InDel-1	TTATCCCCACTTACAGCCCG	GGTAGTACCGCTAGGGGCA	80 bp	Chr 5H 487,228,854
InDel-2	GTTTAGCAGGGAGGCTCGAA	GAATATCTGGCCATGCATGCA	130 bp	Chr 5H 482,479,881
InDel-3	GCGAAACCTTCCTTGCTTCC	TTTGACAAGGTTGGTCTCAAAAA	125 bp	Chr 5H 481,610,815
InDel-4	AGCCAACGGTAGGTACATGC	GCTGCTGCTAGGTCAGAAAGA	122 bp	Chr 5H 487,890,216
InDel-5	CACCCATATTTGTGCATAGGC	TGAGGTTGTGGATTGACGAACA	120 bp	Chr 5H 486,795,826
InDel-6	TCAGGTCCCTTCTTCAGTGA	AGTGCCAGCCAAATGCAGTA	106 bp	Chr 5H 482,690,738
InDel-7	GTTTAAAGCCGGTGAGCGTG	ACGTTGTTATTTATACAACACAGGG	83 bp	Chr 5H 482,623,538
InDel-8	AGGAATTAACAGGACAAATTTAGCA	TCATGGTGAAACATGGTGAATCT	107 bp	Chr 5H 481,623,681
InDel-9	GGTACTACCGCTCGCGAG	GCCAGCAGTAGTACCGCTAG	101 bp	Chr 5H 481,632,966
InDel-10	CAATTGTTCAGGCAAAAATTCA	CTCTCGAGGAAAGCAGGTAATG	139 bp	Chr 5H 481,342,502
InDel-11	GATACTGAAATCTTGCCCATGC	TCAATTGCTTTGTTTGTGGAAC	180 bp	Chr 5H 481,701,251
InDel-12	TGTTGCCCTCTGGTATGAATAG	AATTTCAGTGTCTGAACTATGGG	136 bp	Chr 5H 482,525,089
InDel-13	CCTTCTTCGTCTAGCACCCATA	TGCTCGTACTGAGGTTTCTTGA	175 bp	Chr 5H 486,795,676
InDel-14	ACAGTAGCGCCAGTAATTGTGT	AGTTTCCAGAGTATCACTGCCA	180 bp	Chr 5H 487,123,960
InDel-15	TGTGTGGCAGTTTCTTTAATGG	TGAAGCTTTGTTTTACTGACGG	176 bp	Chr 5H 487,521,136
InDel-16	AACAAGACCTGGAGAGACAAGC	TGCTGCTAGGTCAGAAAGAAAA	138 bp	Chr 5H 487,890,066
InDel-17	CTCCTACCACCCTTTTCACCC	TACTGCAAGAAATCGTACCACC	154 bp	Chr 5H 488,465,184

ISSN: 1471-2164