Fig. 4

Integrative analyses of ChIP-seq and microarray gene expression data sets identify primary ERα and Ctr9 targets. a Venn diagram showing the overlaps between genes with ERα binding increased by E2 treatment, genes with RNAPII binding increased by E2 treatment, and genes with increased expression upon E2 treatment identified from microarray analysis. b Box plots displaying the mRNA expression of primary ERα target gene signature between ERα- (n = 395) and ERα + (n = 1225) breast tumors. The gene expression data were accessed using the Gene Expression-Based Outcome for Breast Cancer Online (GOBO) tool. c Kaplan-Meier survival analysis displaying the relationship between overall survival time and the primary ERα target gene signature in ERα+, lymph node negative (LN-) breast cancer patients. The breast cancer outcome-linked gene expression data were accessed using the GOBO tool. d Venn diagram showing the overlap between genes with RNAPII binding decreased by Ctr9 knockdown and genes with decreased expression upon Ctr9 knockdown identified from microarray analysis in the presence of E2. e Box plots displaying the mRNA expression of Ctr9 target gene signature between ERα- (n = 395) and ERα + (n = 1225) breast tumors. The gene expression data were accessed using the GOBO tool. f Kaplan-Meier survival analysis displaying the relationship between overall survival time and the Ctr9 target gene signature in ERα+, LN- breast cancer patients. The breast cancer outcome-linked gene expression data were accessed using the GOBO tool. g Venn diagram showing the overlap between the 240 Ctr9 target genes noted in (d) and the 279 E2-activated genes noted in (a)